UBC3B (UBE2R2) (N-term) Goat Polyclonal Antibody

CAT#: AP31108PU-N

UBC3B (UBE2R2) (N-term) goat polyclonal antibody, Aff - Purified

Product Images

Paraffin Embedded Human Testis stained with UBE2R2 Antibody at 2 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

Specifications

Product Data

• Applications: ELISA, EM, IHC
• Recommended Dilution: Peptide ELISA: 1/4000 (Detection Limit).
  Western Blot: No signal obtained yet but low background observed in Human Testis and U937 lysates at up to 1 µg/ml.
  Immunoelectron Microscopy: This product stained chromatoid bodies in Rat spermatids.
  Immunohistochemistry on Paraffin Sections: 2-4 μg/ml. In paraffin embedded Human Testis shows textured nuclear staining of the primary spermatocytes.
• Reactivities: Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the N Terminus of the protein sequence according to NP_060281.
• Specificity: Recognizes UBC3B.
• Formulation: Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Stabilizer: 0.5% BSA
  Preservative: 0.02% Sodium Azide
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: ubiquitin conjugating enzyme E2 R2
• Database Link:
  Entrez Gene 54926 Human
  UniProt ID Q712K3
• Background: Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation.
• Synonyms: Ubiquitin-conjugating enzyme E2 R2, Ubiquitin-protein ligase R2, Ubiquitin carrier protein R2, E2-CDC34B, CDC34B, UBC3B

Reference Data

• Protein Pathways: Ubiquitin mediated proteolysis