Grin2b pSer1166 Rabbit Polyclonal Antibody

CAT#: AP26447PU-N

Grin2b pSer1166 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot of rat hippocampal lysate showing specific i mmunolabeling of the ~180k NR2B subunit of the NMDAR phosphorylated at Ser 1166 (Control). Immunolabeling is blocked by preadsorption with the phospho- peptide used as antigen (Peptide), but not by t he corresponding dephospho-peptide (not shown).

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/250.
• Reactivities: Canine, Human, Mouse, Primate, Rat, Xenopus
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho- Ser 1166 of the NR2B subunit of the rat NMDA receptor
• Specificity: Specific for the ~180k NMDAR NR2B subunit phosphorylated at Ser 1166 . Immunolabeling of the NMDA NR2B subunit band is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
• Formulation: 100 μl in 10 mM HEPES (pH 7.5), 150 mM Na Cl, 100 μg per ml BSA and 50% glycerol
  State: Aff - Purified
  State: Liquid Ig fraction
• Purification: Affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glutamate ionotropic receptor NMDA type subunit 2B
• Database Link:
  Entrez Gene 24410 Rat
  UniProt ID Q00960
• Background: The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer’s, epilepsy and ischemic neuronal cell deat h (Grosshans et al., 2002; Wenthold et al., 2003; Carroll and Zukin, 2002). Overexpression of t he NR2B-subunit of the NM DA Receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression (Clayton et al., 2002a; Clayton et al., 2002b). Phosphorylation of Ser 1166 is thought to play an essential role in memory and neuronal development.
• Synonyms: GRIN2B, NMDA Receptor 2B