SFTPB Rabbit Polyclonal Antibody

CAT#: AP26403PU-N

SFTPB rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on frozen sections: The typical starting working dilution is 1:50.
  Immunohistochemistry on paraffin sections: The typical starting working dilution is 1:50.
  Western blot: The typical starting working dilution is 1:50.
• Reactivities: Bovine, Human, Mouse, Porcine
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Human-based 60-amino acid synthetic SP-B polypeptide
• Specificity: This antibody detects SP-B. It recognizes proteins of 42, 25 and 18 kD under nonreducing conditions. The 18 kD form is reduced to 7.7-8 kD in the presence of beta-mercaptoethanol.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein A
• Conjugation: Unconjugated
• Storage:

  Store at 2 - 8 °C.
  

• Stability: Shelf life: one year from despatch.

• Gene Name: surfactant protein B
• Database Link:
  Entrez Gene 6439 Human
  UniProt ID P07988
• Background:

  There are four surfactant-specific proteins, designated surfactant protein A (SP-A), SP-B, SP-C and SP-D respectively. SP-A and SP-D are hydrophilic surfactant proteins and are members of the collectin family. SP-B and SP-C are hydrophobic surfactant proteins and may be the most appropriate indicators for the evolutionary origin of surfactant.
  SP-B is synthesized by the alveolar type II epithelial cells as a 40-42 kD precursor that is subsequently proteolytically processed to 7.8-8 kD. SP-B enhances the spreading and stability of surfactant phospholipids in the alveolus. SP-B is essential for air-breathing in mammals and is therefore largely conserved. SP-B can interact with both phospholipid head groups and fatty chains and is particularly active in enhancing surface active behaviour in endogenous and exogenous lung surfactants. Even low SP-B contents had measurable effects in increasing the adsorption, dynamic surface tension lowering, and/or film respreading of DPPC, mixed synthetic lipids, and column-purified lung surfactant phospholipids. Deficiency of SP-B and other surfactant components is associated with respiratory distress syndrome (RDS) in premature infants and adults with respiratory distress syndrome (ARDS).

• Synonyms: Surfactant protein B, SP-B, SFTP3