Hc Rabbit Polyclonal Antibody

CAT#: AP26372PU-N

Hc rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot. The typical starting working dilution is 1:10.
• Reactivities: Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Specificity: This antibody detects C5.
• Formulation: PBS
  State: Purified
  State: Liquid 0.2 µm filtered Ig fraction
  Stabilizer: 0.1% bovine serum albumin
  Preservative: 0.02% sodium azide
• Concentration: lot specific
• Purification: Protein A
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C.
  
• Stability: Shelf life: one year from despatch.
• Gene Name: hemolytic complement
• Database Link:
  Entrez Gene 15139 Mouse
  UniProt ID P06684
• Background:

  
  C5 is involved in the activation of the lythic pathway within the complement system which is an important factor in innate immunity. The complement pathways can be divided in the activation pathways and lytic pathway. The activation pathways lead via C3 to the cleavage of the fifth complement component C5 into C5a and C5b.
  C5a induces smooth muscle contraction, increases vascular permeability, causes degranulation of mast cells and basophils, and release of lysosomal enzymes. In addition C5a stimulates the directed migration of neutrophils, eosinophils, basophils and monocytes. Studies indicate the modulation of the acute-phase response in liver and an overall immune response by inducing synthesis of cytokines such as TNF-alpha, IL-1beta, IL-6 and IL-8.
  C5b initiates the assembly of the membrane attack complex (MAC) that mediates cytolysis.

• Synonyms: CPAMD4, Complement component 5