IL17D Rabbit Polyclonal Antibody

CAT#: AP23788BT-N

IL17D rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Sandwich ELISA: To detect Human IL17D by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Purified Anti-Human IL17D (AP23788PU-N or AP23788PU-S) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL17D.
  Direct ELISA: To detect Human IL17D by Direct ELISA (using 100μl/well antibody solution) a concentration of 0.2-1.0 μg/ml of this antibody is required. This antigen Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human IL17D.
  Western Blot: To detect Human IL17D by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IL17D is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure recombinant Human IL-17D.
• Specificity: Recognizes Interleukin-17D / IL17D
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing an immobilized Human 17D matrix
• Conjugation: Biotin
• Storage: Store the lyophilized antibody at -20°C.
  Following reconstitution it is stable for two weeks at 2-8°C.
  Frozen aliquots are stable for 6 months when stored at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch
• Gene Name: interleukin 17D
• Database Link:
  Entrez Gene 53342 Human
  UniProt ID Q8TAD2
• Background: The originally described IL-17 protein, now known as IL-17A, is a homodimer of two 155 amino acid chains, secreted by activated T-cells and acts on stromal cells to induce production of proinflammatory and hematopoietic bioactive molecules. Today, IL-17 represents a family of structurally-related cytokines that share a highly conserved C-terminal region and differ from one another in their N-terminal segments and in their distinct biological roles. The six known members of this family, IL-17A through IL-17F, are secreted as disulfide-linked homodimers. IL-17D has the ability to stimulate the production of IL-6, IL-8 and GM-CSF and inhibits hemopoiesis of myeloid progenitor cells in colony forming assays.
• Synonyms: IL-17D, IL27, Interleukin-27

Reference Data

• Protein Families: Druggable Genome, Secreted Protein