CCL27 Rabbit Polyclonal Antibody

CAT#: AP23787PU-S

CCL27 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot (Reduced) using ESkine / CCL27 Antibody Cat.-No AP23787PU

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  Western Blot (Unreduced) using ESkine / CCL27 Antibody Cat.-No AP23787PU

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  Sandwich ELISA using ESkine / CCL27 Antibody Cat.-No AP23787PU

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  Immunofluorescence: This antibody stained HeLa cells. The primary antibody was incubated at 3.0 mg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Information and photo are courtesy of the Cell Profiling group, SciLifeLab Stockholm.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: Sandwich ELISA: To detect Human CTACK by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTACK (Cat.-No AP23787BT-N or AP23787BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human CTACK.
  Western Blot: To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
  Immunofluorescence: This antibody stained HeLa cells. The primary antibody was incubated at 3.0 µg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure recombinant Human CTACK.
• Specificity: Recognizes Human CTACK.
  Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  State: Aff - Purified
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method: Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
• Purification: Affinity Chromatography employing an immobilized Human CTACK matrix
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: C-C motif chemokine ligand 27
• Database Link:
  Entrez Gene 10850 Human
  UniProt ID Q9Y4X3
• Background: CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues.
• Synonyms: C-C motif chemokine 27, Small-inducible cytokine A27, ILC, SCYA27, Skinkine, CTACK

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Cytokine-cytokine receptor interaction