CCL27 Rabbit Polyclonal Antibody
Other products for "CCL27"
Specifications
Product Data | |
Applications | ELISA, IF, WB |
Recommended Dilution | Sandwich ELISA: To detect Human CTACK by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human CTACK (Cat.-No AP23787BT-N or AP23787BT-S) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human CTACK. Western Blot: To detect Human CTACK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human CTACK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunofluorescence: This antibody stained HeLa cells. The primary antibody was incubated at 3.0 µg/ml overnight at 4°C followed by a fluorescent labeled secondary antibody. Information courtesy of the Cell Profiling group, SciLifeLab Stockholm. |
Reactivities | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | Highly pure recombinant Human CTACK. |
Specificity | Recognizes Human CTACK. Other species not tested. |
Formulation | PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction |
Reconstitution Method | Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity Chromatography employing an immobilized Human CTACK matrix |
Conjugation | Unconjugated |
Storage | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Stability | Shelf life: one year from despatch. |
Gene Name | C-C motif chemokine ligand 27 |
Database Link | |
Background | CTACK is a keratinocyte-derived CC chemokine which signals through the CCR10 receptor. Both CTACK and CCR10 are expressed in normal and irritated epithelial cells. CTACK selectively attracts CLA+ T-cells and directs them into the skin. CTACK contains the four highly conserved cysteine residues present in most CC chemokines. The mature protein contains 88 amino acid residues. |
Synonyms | C-C motif chemokine 27, Small-inducible cytokine A27, ILC, SCYA27, Skinkine, CTACK |
Reference Data | |
Protein Families | Druggable Genome |
Protein Pathways | Chemokine signaling pathway, Cytokine-cytokine receptor interaction |
Documents
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