TNFRSF1A (195-211) Rabbit Polyclonal Antibody

CAT#: AP23346PU-N

TNFRSF1A (195-211) rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry on Human Mammary Paraffin Section Tissue using CD120a / TNFR1 antibody Cat.-No AP23346PU-N.

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  Western blotting analysis using CD120a / TNFR1 antibody Cat.-No AP23346PU-N. Lane 1: Recombinant Human TNFR1 Protein 10ng. Lane 2: Recombinant Human TNFR1 Protein 5ng. Lane 3: Recombinant Human TNFR1 Protein 2.5ng.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 0.1-0.5 μg/ml.
  Immunohistochemistry on Paraffin Sections: 0.5-1 μg/ml.
  By Heat: Boiling the paraffin sections in 10mM citrate buffer, pH6.0, for 20mins is required for the staining of formalin/paraffin sections.
• Reactivities: Goat, Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequencein the middle region of Human TNFR1 (aa 195-211).
• Specificity: This antibody detects CD120a / TNFR1 (195-211). No cross reactivity with other proteins.
• Formulation: 0.9 mg NaCl, 0.2 mg Na₂HPO₄
  State: Aff - Purified
  State: Lyophilized Ig fraction
  Stabilizer: 5 mg BSA
  Preservative: 0.05 mg Thimerosal, 0.05 mg Sodium Azide
• Reconstitution Method: 0.2 ml of distilled water will yield a concentration of 0.5 mg/ml.
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tumor necrosis factor receptor superfamily member 1A
• Database Link:
  Entrez Gene 21937 MouseEntrez Gene 25625 RatEntrez Gene 7132 Human
  UniProt ID P19438
• Background: Tumor necrosis factor receptor 1(TNFR1), a potent cytokine, elicits a broad spectrum of biologic responses which are mediated by binding to a cell surface receptor. Its gene is located on 12p13.2. The coding region and the 3-prime untranslated region of TNFR1 are distributed over 10 exons. There are 2 different proteins that serve as major receptors for TNF-alpha, one associated with myeloid cells and one associated with epithelial cells. Additionally, TNFR1 associates with the MADD protein through a death domain-death domain interaction. MADD provides a physical link between TNFR1 and the induction of mitogen-activated protein (MAP) kinase (e.g., ERK2) activation and arachidonic acid release. TNFR1-induced apoptosis involves 2 sequential signaling complexes. Complex I, the initial plasma membrane-bound complex, consists of TNFR1, the adaptor TRADD, the kinase RIP1, and TRAF2 and rapidly signals activation of NF-kappa-B. In a second step, TRADD and RIP1 associate with FADD and caspase-8, forming a cytoplasmic complex, complex II.
• Synonyms: Tumor necrosis factor receptor 1, TNF-R1, TNF-RI, TNFR-I, p55, p60, Tnfrsf1a

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transcription Factors, Transmembrane
• Protein Pathways: Adipocytokine signaling pathway, Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Apoptosis, Cytokine-cytokine receptor interaction, MAPK signaling pathway