HRH3 (C-term) Rabbit Polyclonal Antibody

CAT#: AP23342PU-N

HRH3 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

HRH3 Polyclonal Antibody

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot 0.75 μg/ml.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: A synthetic peptide corresponding to a sequence at the C-terminal of human HRH3
• Specificity: This antibody detects Histamine H3 receptor (C-term). No cross reactivity with other proteins.
• Formulation: 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 0.2ml of distilled water will yield a concentration of 500μg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: histamine receptor H3
• Database Link:
  Entrez Gene 85268 RatEntrez Gene 99296 MouseEntrez Gene 11255 Human
  UniProt ID Q9Y5N1
• Background: The histamine receptor H3 (HRH3) is a presynaptic autoreceptor on histamine neurons in the brain and a presynaptic heteroreceptor in nonhistamine-containing neurons in both the central and peripheral nervous systems1. The deduced 445-amino acid HRH3 protein contains 7 predicted transmembrane domains. And it shares 22% and 21.4% amino acid sequence homology with the H1 (HRH1) and H2 (HRH2) receptors, respectively. The expression of recombinant HRH3 in a variety of cell lines conferred an ability to inhibit adenylate cyclase in response to histamine, but not to acetylcholine or any other biogenic amine. Additionally, HRH3 was most notably observed throughout the thalamus, the ventromedial hypothalamus, and the caudate nucleus. Strong expression was also seen in layers II, V,and VIb of the cerebral cortex, in the pyramidal layers of the hippocampus, and in olfactory tubercle.
• Synonyms: HH3R, GPCR97, G-protein coupled receptor 97

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction