GJA1 (C-term) Rabbit Polyclonal Antibody

CAT#: AP23305PU-N

GJA1 (C-term) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of rat cardiac muscletissue lysis using Connexin 43 antibody

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  Immunohistochemical analysis of paraffin embedded rat cardiac muscle section, staining Connexin 43 in cytoplasm, DAB chromogenic reaction

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: At 2µg/ml with the appropriate system to detect Connexin 43 in cells and tissues.
  Immunohistochemistry on paraffin sections: At 1-2µg/ml to detect Connexin 43 in formalin fixed and paraffin embedded tissues.
  Immunocytochemistry.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peptide mapping at the C-terminus of human Connexin 43
• Specificity: This antibody detects GJA1 / Cx43 at C-term. No cross reactivity with other proteins.
• Formulation: 50% glycerol, 0.9mg NaCl, 0.2mg Na2HPO4
  State: Aff - Purified
  State: Lyophilized Ig fraction
• Reconstitution Method: 1.2% sodium acetate or neutral PBS. If 0.5ml of PBS is used, the antibody concentration will be 100μg/ml.
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: gap junction protein alpha 1
• Database Link:
  Entrez Gene 14609 MouseEntrez Gene 24392 RatEntrez Gene 2697 Human
  UniProt ID P17302
• Background: Connexins 43 (Cx43), also called GAP Junction Protein, alpha-1(GJA1). Connexin 43 is a member of the connexin gene family which abundantly expressed in the heart and liver and was mapped to 6q21-q23.2. Connexin43, the major protein of gap junctions in the heart, is targeted by several protein kinases that regulate myocardial cell-cell coupling. Mutations in the connexin43 gap-junction gene,which lead to abnormally regulated cell-cell communication, are associated with visceroatrial heterotaxia. Cx43 must also play a critical role in the physiology of hearing, presumably by participating in the recycling of potassium to the cochlear endolymph.
• Synonyms: Gap junction alpha-1 protein, Connexin-43, GJAL

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Gap junction