Glutathione Peroxidase 4 (GPX4) (184-196) Goat Polyclonal Antibody

CAT#: AP22541PU-N

Glutathione Peroxidase 4 (184-196) goat polyclonal antibody, Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Product Images

Antibody (0.1 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  Human Testis (formalin-fixed, paraffin-embedded) stained with GPX4 antibody at 2.5 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: IHC
  IHC-P (2.5 µg/ml)
  Peptide-ELISA (1:16000)
  WB (0.1 - 0.3 µg/ml)
• Reactivities: Human, Monkey
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence C-EEPLVIEKDLPHY, from the C-Terminus of protein sequence according to NP_002076.2NP_001034937.1.
• Specificity: Human GPX4. This antibody is expected to recognise isoform A (NP_002076.2) and isoform C (NP_001034937.1).
• Formulation: TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Aliquot and store at -20°C. Avoid freeze-thaw cycles.
• Stability: 12 months from date of despatch
• Gene Name: glutathione peroxidase 4
• Database Link:
  Entrez Gene 2879 Human
  UniProt ID P36969
• Background: MCSP antibody LS-B3246 is an unconjugated goat polyclonal antibody to MCSP (GPX4) (aa184-196) from human. It is reactive with human and monkey. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues. Cited in 2 publications.
• Synonyms: GPX4, GPx-4, MCSP, SnPHGPx, Phospholipid hydroperoxidase, SnGPx, Glutathione peroxidase 4, GSHPx-4, PHGPx
• Note: Immunohistochemistry: LS-B3246 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B3246 was determined to be 2.5 ug/ml.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Arachidonic acid metabolism, Glutathione metabolism