GAD67 (GAD1) (526-537) Goat Polyclonal Antibody

CAT#: AP22514PU-N

GAD67 (GAD1) (526-537) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (1 ug/ml) staining of Mouse Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence

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  Human Brain, Cerebellum (formalin-fixed, paraffin-embedded) stained with GAD1 antibody at 4 ug/ml followed by biotinylated anti-goat IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1/8000.
  Immunohistochemistry on Paraffin Sections: 4 µg/ml.
  Western Blot: 0.3 - 1 µg/ml.
• Reactivities: Bat, Bovine, Canine, Equine, Human, Mouse, Opossum, Porcine, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Synthetic peptide from an internal region of human GAD1 / GAD67 (NP_000808.2)
• Specificity: This antibody detects GAD1 / GAD67 (Internal).
  It is expected to recognize isoform GAD67. There is no cross-reactivity expected with GAD2.
• Formulation: Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA
  State: Aff - Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to three months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: glutamate decarboxylase 1
• Database Link:
  Entrez Gene 14415 MouseEntrez Gene 24379 RatEntrez Gene 2571 Human
  UniProt ID Q99259
• Background: GAD1 / GAD67 is one of several forms of glutamic acid decarboxylase, identified as a major autoantigen in insulin-dependent diabetes. The enzyme encoded is responsible for catalyzing the production of gamma-aminobutyric acid from L-glutamic acid. A pathogenic role for this enzyme has been identified in the human pancreas since it has been identified as an autoantigen and an autoreactive T cell target in insulin-dependent diabetes. This gene may also play a role in the stiff man syndrome.
• Synonyms: Glutamate decarboxylase 1, GAD-67

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alanine, aspartate and glutamate metabolism, beta-Alanine metabolism, Butanoate metabolism, Metabolic pathways, Taurine and hypotaurine metabolism, Type I diabetes mellitus