MID1IP1 Goat Polyclonal Antibody

CAT#: AP22415PU-N

MID1IP1 goat polyclonal antibody, Aff - Purified

Product Images

HEK293 lysate (10ug protein in RIPA buffer) overexpressing Human MID1IP1 with C-terminal MYC tag probed with (1 ug/ml) in Lane A and probed with anti-MYC Tag (1/1000) in lane C. Mock-transfected HEK293 probed with (1 mg/ml) in Lane B. Primary incubations were for 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Peptide ELISA: Limit Dlution: 1/8000.
  Western blot: 0.2-1.0 µg/ml. In transfected HEK293 transiently expressing full-length Human MID1IP1 (myc and DYKDDDDK tagged), a band of approx. 28kDa was observed. No bands were observed in mock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_067065.1.
• Specificity: Recognizes MIG12
• Formulation: Tris saline, pH~7.3 with 0.02% Sodium Azide and 0.5% BSA
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: MID1 interacting protein 1
• Database Link:
  Entrez Gene 58526 Human
  UniProt ID Q9NPA3
• Synonyms: STRAIT11499, MID1IP1, MIG12