DLL4 Rabbit Polyclonal Antibody

CAT#: AP21750BT-S

DLL4 rabbit polyclonal antibody, Biotin

Product Images

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Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: Direct ELISA: To detect Human sDLL-4 by Direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25–1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2–0.4 ng/well of recombinant hsDLL-4.
  Sandwich ELISA: To detect Human sDLL-4 by Sandwich ELISA (using 100μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Affinity purified Anti-Human sDLL-4 (Cat.-No AP21750PU-N or AP21750PU-S) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human sDLL-4.
  Western Blot: To detect Human sDLL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsDLL-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Highly pure HEK293 cells derived Recombinant Human sDLL-4 (Cat.-No AR31113PU-N).
• Specificity: Recognizes Human sDLL-4. Other species not tested.
• Formulation: PBS, pH 7.2 without preservatives
  Label: Biotin
  State: Lyophilized (sterile filtered) purified Ig fraction
• Reconstitution Method:

  Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.

• Purification: Affinity Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: delta like canonical Notch ligand 4
• Database Link:
  Entrez Gene 54567 Human
  UniProt ID Q9NR61
• Background:

  DLL4 is a homolog of the Drosophila delta protein. The delta gene family encodes Notch ligands that are characterized by a DSL domain, EGF repeats, and a transmembrane domain.

• Synonyms: Delta 4, Drosophila Delta homolog 4

Reference Data

• Protein Families: Cancer stem cells, Druggable Genome, ES Cell Differentiation/IPS, Stem cell relevant signaling - DSL/Notch pathway, Transmembrane
• Protein Pathways: Notch signaling pathway