Phospholipase D Rabbit Polyclonal Antibody

CAT#: AP21377AF-N

Phospholipase D rabbit polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques). To prepare an insoluble Immuno-Affinity adsorbent. For labelling with a marker of the customer's own choice.
  Working Dilutions: vary widely, but may be between 1/1000 and 1/7000.
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Phospholipase D isolated and purified from Cabbage. Freund's complete adjuvant is used in the first step of the immunization procedure.
• Specificity: IgG fraction of polyclonal Rabbit antiserum to Phospholipase D from Cabbage.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  State: Azide Free
  State: Lyophilized hyperimmune IgG fraction
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Unconjugated
• Storage: Prior to and following reconstitution store the antibody at 2-8°C for one month or at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID O82549
• Background: Phospholipase D (EC=3.1.4.4) is an enzyme involved in lipid degradation by hydrolyzing glycerol phospholipids. Involved in many cellular process including phytohormone action, membrane deterioration, senescence and more.
• Synonyms: Phospholipase D alpha 1, Phospholipase D alpha 2, Choline phosphatase 1, Choline phosphatase 2, PLD1, PLD-1, PLD2, PLD-2