PGK1 Rabbit Polyclonal Antibody

CAT#: AP21371BT-N

PGK1 rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: Can be used in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and immunofluorescence or histochemical techniques).
  Recommended working dilutions: vary widely, but may be between 1/1,000 and 1/30,000.
• Reactivities: Bakers Yeast
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: 3-Phosphoglyceric phosphokinase isolated and purified from baker's yeast.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: 3-Phosphoglyceric Phosphokinase from Baker’s Yeast.
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, Single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized hyperimmune IgG fraction
  Molar radio: Biotin/IgG ~6.1
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Storage: Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P00560
• Synonyms: Phosphoglycerate kinase, YCR012W, YCR12W