Horseradish Peroxidase / HRP Rabbit Polyclonal Antibody

CAT#: AP21360PU-N

Horseradish Peroxidase / HRP rabbit polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity adsorbent, for labelling with a marker of
  the customer’s own choice.
  Working dilutions in non-precipitating antibody-binding techniques: 1/1,000-1/10,000.
• Reactivities: Horsereddish
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peroxidase isolated and purified from Horseradish.
  Freund’s complete adjuvant is used in the first step of the immunization procedure.
• Specificity: The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins
  State: Aff - Purified
  State: Lyophilized purified IgG fraction
• Reconstitution Method: Restore by adding 0.5 ml of sterile distilled water
• Concentration: lot specific
• Purification: Solid Phase Affinity Chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
• Stability: Shelf life: one year from despatch.
• Background: Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems.
  HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.