Collagenase Rabbit Polyclonal Antibody
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Specifications
Product Data | |
Applications | ELISA, ID, IF, IP, R, WB |
Recommended Dilution | This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques), to prepare an insoluble immuno-affinity Adsorbent, for labelling with a Marker of the customer's own choice. Working dilutions in non-precipitating antibody-binding techniques: 1/10,000-1/50,000. |
Reactivities | Clostridium histolyticum |
Host | Rabbit |
Isotype | IgG |
Clonality | Polyclonal |
Immunogen | Collagenase is isolated and purified from Clostridium histolyticum (containing the different isoforms). Freund's complete adjuvant is used in the first step of the immunization procedure. |
Specificity | Collagenase from Clostridium histolyticum (containing the different isoforms). The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition. Cross-reactivities against enzymes of other sources may occur but have not been determined. |
Formulation | PBS, pH 7.2 stabilized with Dextran without preservatives and foreign proteins State: Aff - Purified State: Lyophilized purified Hyperimmune IgG fraction |
Reconstitution Method | Restore by adding 0.5 ml of sterile distilled water |
Purification | Solid Phase Affinity Chromatography |
Conjugation | Unconjugated |
Storage | Store the antibody lyophilized at 2-8°C and reconstituted at 2-8°C for one week or (in aliquots) at -20°C for longer. If a slight precipitation occurs upon storage, this should be removed by centrifugation. |
Stability | Shelf life: one year from despatch |
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.