Collagenase Rabbit Polyclonal Antibody

CAT#: AP21244BT-N

Collagenase rabbit polyclonal antibody, Biotin

Specifications

Product Data

• Applications: ELISA, ID, IF, IP, R, WB
• Recommended Dilution: This product is intended for use in precipitating and non-precipitating antibody-binding assays (such as e.g., ELISA and Western blotting and Immunofluorescence or Histochemical techniques).
  Working Dilutions in non-precipitating antibody-binding techniques: 1/5,000-1/100,000.
• Reactivities: Clostridium histolyticum
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Collagenase is isolated and purified from Clostridium histolyticum (containing the different isoforms).
  Freund’s complete adjuvant is used in the first step of the immunization
• Specificity: Collagenase from Clostridium histolyticum (containing the different isoforms).
  The reagents were evaluated for potency, purity and specificity using most or all of the following techniques: Immunoelectrophoresis, Cross-Immunoelectrophoresis, single Radial Immunodiffusion (Ouchterlony), block titration, ELISA, Immunoblotting and Enzyme Inhibition.
  Cross-reactivities against enzymes of other sources may occur but have not been determined.
• Formulation: PBS, pH 7.2 without preservatives and foreign proteins
  Label: Biotin
  State: Lyophilized Hyperimmune IgG fraction
  Molar radio: Biotin/ IgG ~2.2
• Reconstitution Method: Restore by adding 1.0 ml of sterile distilled water
• Purification: Ammonium Sulphate Precipitation and Ion Exchange Chromatography
• Conjugation: Biotin
• Storage: Store lyophilized at 2-8°C for 6 months or at -20°C long term.
  After reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C long term.
  If a slight precipitation occurs upon storage, this should be removed by centrifugation.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.