FOXO1 Rabbit Polyclonal Antibody

CAT#: AP20978PU-N

FOXO1 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of FKHR/FOXO1A antibody (Cat.-No.: AP20978PU-N) in extracts from NIH/3T3 cells.

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  Immunohistochemistry (IHC) analyzes of FKHR/FOXO1A antibody (Cat.-No.: AP20978PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of FKHR/FOXO1A protein.
  (region surrounding Ser262)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 70 to 82 kDa
• Gene Name: forkhead box O1
• Database Link:
  Entrez Gene 56458 MouseEntrez Gene 84482 RatEntrez Gene 2308 Human
  UniProt ID Q12778
• Background: FKHR (for forkhead in rhabdomyosarcoma) and FKHRL1 are members of the forkhead family of transcription factors. Transcriptional activation of FKHR proteins is regulated by the serine/threonine kinase Akt1, which phosphorylates FKHRL1 and results in FKHRL1 associating with 14-3-3 proteins and being retained in the cytoplasm. Induction of apoptosis or withdrawal of growth factors stimulates dephosphorylation and nuclear translocation of FKHR proteins, leading to FKHR-induced gene-specific transcriptional activation. FKHR, also designated forkhead box protein O1A (FOXO1), is an ubiquitously expressed protein that shuttles between the cytoplasm and nucleus. Genetic mutations in FKHR genes, including the t(2;13) and t(1;3) translocations, are commonly found in alveolar rhabdomyosarcomas.
• Synonyms: Forkhead Box Protein O1, FOXO1, FKHR

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Insulin signaling pathway, Pathways in cancer, Prostate cancer