beta Catenin (CTNNB1) pThr41/pSer45 Rabbit Polyclonal Antibody

CAT#: AP20927PU-N

beta Catenin (CTNNB1) pThr41/pSer45 rabbit polyclonal antibody, Aff - Purified

Product Images

Immunohistochemistry (IHC) analyz es of p-beta-catenin (pThr41/pSer45) antibody (Cat.-No.: AP20927PU-N) in paraffin-embedded human plung adenocarcinoma tissue.

Specifications

Product Data

• Applications: IHC
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-beta-catenin protein only when phosphorylated at Thr41/S45.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 92 kDa
• Gene Name: catenin beta 1
• Database Link:
  Entrez Gene 12387 MouseEntrez Gene 84353 RatEntrez Gene 1499 Human
  UniProt ID P35222
• Background: The catenins, alpha, beta and gamma, are proteins that bind to the highly conserved, intracellular cytoplasmic tail of E-cadherin. Together, the catenin/cadherin complexes play critical roles in mediating cellular adhesion. beta-catenin associates with the cytoplasmic portion of E-cadherin, which is necessary for the function of E-cadherin as an adhesion molecule. beta-catenin also forms complexes with the tumor suppressor protein APC. Amino acid alterations at residues around Ser 33, one of the targets for phosphorylation of glycogen synthase kinase-3beta, result in accumulation of the beta-catenin protein in the cytoplasm and nucleus. Pin1 is a novel regulator of beta-catenin signaling that directly binds a phosphorylated Ser-Pro motif next to the APC-binding site in beta-catenin, inhibiting the interaction with APC and increasing beta-catenin translocation into the nucleus. Thus, Pin1 overexpression may contribute to the upregulation of beta-catenin in tumors such as breast cancer.
• Synonyms: CTNNB1, CTNNB, Beta-catenin

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Basal cell carcinoma, Colorectal cancer, Endometrial cancer, Focal adhesion, Leukocyte transendothelial migration, Melanogenesis, Pathogenic Escherichia coli infection, Pathways in cancer, Prostate cancer, Thyroid cancer, Tight junction, Wnt signaling pathway