GSK3 alpha (GSK3A) pSer21 Rabbit Polyclonal Antibody

CAT#: AP20890PU-N

GSK3 alpha (GSK3A) pSer21 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-GSK3alpha antibody (Cat.-No.: AP20890PU-N) in extracts from ovary cancer.

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  Immunohistochemistry (IHC) analyzes of p-GSK3alpha antibody (Cat.-No. AP20890PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-GSK3alpha protein only when phosphorylated at Ser21.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 46,51 kDa
• Gene Name: glycogen synthase kinase 3 alpha
• Database Link:
  Entrez Gene 50686 RatEntrez Gene 606496 MouseEntrez Gene 2931 Human
  UniProt ID P49840
• Background: Glycogen synthase kinase-3 α and β (GSK-3alpha, beta) are serine/threonine kinases that regulate metabolic enzymes and transcription factors, which are responsible for coordinating processes such as glycogen synthesis and cell adhesion. GSK-3beta activity is also required for nuclear activity of Rel dimers, which mediate an anti-apoptotic response to TNFalpha in mice. GSK-3 catalytic kinase activity is controlled through differential phosphorylation of serine/threonine residues, which have an inhibitory effect, and tyrosine residues, which have an activating effect. Growth factor stimulation of mammalian cells expressing GSK-3alpha and GSK-3beta induces phosphorylation of Ser 21 and Ser 9, respectively through a phosphatidylinositol 3-kinase (PI 3-kinase)-protein kinase B (PKB) dependent pathway, thereby enhancing proliferative signals. Additionally, GSK-3 physically associates with cAMP-dependent protein kinase A (PKA), which phosphorylates Ser 21 of GSK-3alpha or Ser 9 of GSK-3beta and inactivates both forms.
• Synonyms: Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway