CAMK2A pThr286 Rabbit Polyclonal Antibody

CAT#: AP20855PU-N

CAMK2A pThr286 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-CaMKIIalpha/delta antibody in extracts from K562 cells.

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  Immunohistochemistry (IHC) analyzes of p-CaMKIIalpha/delta antibody in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic phosphopeptide derived from human CaMKIIα around the phosphorylation site of Threonine 286.
• Specificity: This antibody detects endogenous levels of CaMKIIalpha/delta protein only when phosphorylated at Thr286
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE)
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 54 kDa
• Gene Name: calcium/calmodulin dependent protein kinase II alpha
• Database Link:
  Entrez Gene 815 Human
  UniProt ID Q9UQM7
• Background: The Ca²⁺/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is an ubiquitously expressed serine/threonine protein kinase that is activated by Ca²⁺ and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes, designated α, β, γ and δ, which may or may not be co-expressed in the same tissue type. CaMKIIα is autophosphorylated on Thr286 upon the binding of the Ca²⁺/CaM complex to the autoinhibitory domain of CaMKII. This process is called Ca²⁺/CaM trapping, which is thought to be involved in the synaptic encoding of information.
• Synonyms: CAMKA, KIAA0968, CaM-kinase II alpha chain, CaM kinase II subunit alpha, CaMK-II subunit alpha