HSP27 (HSPB1) pSer78 Rabbit Polyclonal Antibody

CAT#: AP20847PU-N

HSP27 (HSPB1) pSer78 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of p-HSP27 antibody (Cat.-No.: AP20847PU-N) in extracts from HeLa Ca2+ cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of p-HSP27 protein only when phosphorylated at Ser78.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 27 kDa
• Gene Name: heat shock protein family B (small) member 1
• Database Link:
  Entrez Gene 3315 Human
  UniProt ID P04792
• Background: HSP 27 is a constitutively expressed cytoplasmic protein that co-localizes to the nucleus upon stress-induced insult. The intracellular concentration of HSP 27 increases several-fold after heat shock and other metabolic stresses, and is closely associated with the acquisition of thermotolerance. In addition to heat shock, cytokines and hormones are among the factors that stimulate the synthesis of HSP 27. MAP kinaseactivated protein kinase-2 phosphorylates HSP 27 on Serine residues Ser 15, Ser 78 and Ser 82, which are phosphorylated in vivo in response to growth factors and heat shock. Ser 15, Ser 78 and Ser 82 occur in the sequence motif RXXS, which is recognized by ribosomal protein S6 kinase II.
• Synonyms: Heat shock protein beta-1, Heat shock 27 kDa protein, HSP28, 28 kDa heat shock protein, SRP27, HSP25

Reference Data

• Protein Pathways: MAPK signaling pathway, VEGF signaling pathway