PKC theta (PRKCQ) pSer676 Rabbit Polyclonal Antibody

CAT#: AP20801PU-N

PKC theta (PRKCQ) pSer676 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of p-PKC ? antibody (Cat.-No.: AP20801PU-N) at 1/500 dilution Lane 1:Hela whole cell lysate treated with UV Lane 2:sp2/0 whole cell lysate treated with UV Lane 3:PC12 whole cell lysate treated with UV

• 

  Immunohistochemistry (IHC) analyzes of p-PKC theta antibody (Cat.-No.: AP20801PU-N) in paraffin-embedded human breast carcinoma tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
  Immunofluorescence: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of PKC theta protein only when phosphorylated at Ser676.
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 80 kDa
• Gene Name: protein kinase C theta
• Database Link:
  Entrez Gene 18761 MouseEntrez Gene 85420 RatEntrez Gene 5588 Human
  UniProt ID Q04759
• Background: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into many different isoforms (alpha, betaI, betaII, gamma, delta, epsilon, zeta, eta, theta, iota, lambda, my and ny). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies.
• Synonyms: PKC theta, PKC-theta, nPKC-theta, Protein kinase C theta type

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, T cell receptor signaling pathway, Tight junction, Vascular smooth muscle contraction