MARCKS Rabbit Polyclonal Antibody

CAT#: AP20755PU-N

MARCKS rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analyzes of MARCKS antibody (Cat.-No.: AP20755PU-N) in extracts from HeLa cells.

• 

  Immunohistochemistry (IHC)analyzes of MARCKS antibody (Cat.-No.: AP20755PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of MARCKS protein.
  (region surrounding Arg157)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 32, 75, 87 kDa
• Gene Name: myristoylated alanine rich protein kinase C substrate
• Database Link:
  Entrez Gene 17118 MouseEntrez Gene 25603 RatEntrez Gene 4082 Human
  UniProt ID P29966
• Background: Myristoylated alanine-rich protein kinase C substrate (MARCKS), also designated 80K or 80K-L, has been identified as a major cellular substrate for protein kinase C. Human MARCKS is a 332 amino acid protein with a calculated molecular weight of 31.534 kDa; however, it has been shown to run at 80-87 kDa on Western blot. The plasma membrane bound protein dissociates from the membrane upon phosphorylation by various PKC isoforms. In NIH/3T3 fibroblasts, PKC alpha and PKC epsilon, but not PKC delta, are responsible for MARCKS phosphorylation. MARCKS has been found to bind calmodulin, Actin and Synapsin and is a filamentous (F) Actin crosslinking protein.
• Synonyms: Myristoylated alanine-rich C-kinase substrate, MACS, PRKCSL

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Fc gamma R-mediated phagocytosis