IL12A Rabbit Polyclonal Antibody

CAT#: AP20613PU-N

IL12A rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of IL-12A antibody (Cat.-No.: AP20613PU-N) in extracts from HeLa cells.

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  Immunohistochemistry (IHC) analyzes of IL-12A antibody (Cat.-No.: AP20613PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on paraffin sectins: 1/50 - 1/200.
  Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of IL-12A protein.
  (region surrounding Thr65)
• Formulation: PBS, pH 7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 25 kDa
• Gene Name: interleukin 12A
• Database Link:
  Entrez Gene 16159 MouseEntrez Gene 84405 RatEntrez Gene 3592 Human
  UniProt ID P29459
• Background: IL-12 is secreted by macrophages and human B-lymphoblastoid cells in response to antigenic stimulation. This IL is responsible for the differentiation of naive CD4+ T cells into type 1 helper T cells that produce interferon-γ (IFN-γ). It also activates production of tumor necrosis factor α (TNFα) from T and natural killer (NK) cells, and it inhibits IL-4 mediated suppression of IFN-γ. IL-12 also has anti-angiogenic activity since the production of IFN-γ increases the production of inducible protein-10 (IP-10). IL-12 p40 represents a subunit of IL-12 that is induced by several pathogens. IL-12 p70 is a subunit of IL-12 that is controlled through both the p35 and p40 genes in a cell type-specific fashion.
• Synonyms: IL-12A, IL-12 subunit p35, NKSF1, CLMF p35

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus