CD3E Rabbit Polyclonal Antibody

CAT#: AP20600PU-N

CD3E rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of CD3-epsilon antibody (Cat.-No.: AP20600PU-N) in extracts from K562 cells.

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  Immunohistochemistry (IHC) analyzes of CD3-epsilon antibody (Cat.-No.: AP20600PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Synthetic peptide, corresponding to amino acids 31-80 of Human CD3-ε.
• Specificity: This antibody detects endogenous levels of CD3-epsilon protein.
  (region surrounding Asp71)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 21 kDa
• Gene Name: CD3e molecule
• Database Link:
  Entrez Gene 12501 MouseEntrez Gene 315609 RatEntrez Gene 916 Human
  UniProt ID P07766
• Background: The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (Cluster of Differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of gamma and epsilon chains (gamma-epsilon), a heterodimer of delta and epsilon chains (delta-epsilon) and a homodimer of two zeta chains (zeta-zeta) or a heterodimer of zeta and eta chains (zeta-eta). The zeta and eta chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The gamma, epsilon and delta chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the zeta chain contains three consecutive copies of the same motif.
• Synonyms: T3/Leu-4

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage, Primary immunodeficiency, T cell receptor signaling pathway