DNA Ligase I (LIG1) Rabbit Polyclonal Antibody

CAT#: AP20546PU-N

DNA Ligase I (LIG1) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of DNA Ligase I antibody (Cat.-No.: AP20546PU-N) in extracts from HT-29 cells.

• 

  Immunohistochemistry (IHC) analyzes of DNA Ligase I antibody (Cat.-No.: AP20546PU-N) in paraffin-embedded human brain tissue.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunohistochemistry on paraffin sections: 1/50 - 1/200.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of DNA Ligase I protein.
  (region surrounding Glu139)
• Formulation: Phosphate buffered saline (PBS), pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
  Preservative: 0.05% sodium azide
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: 102,125 kDa
• Gene Name: DNA ligase 1
• Database Link:
  Entrez Gene 3978 Human
  UniProt ID P18858
• Background: Eukaryotic DNA ligases are ATP-dependent enzymes that catalyse the joining of single and double-strand DNA breaks, which is an essential final step in DNA replication, recombination and repair. Four biochemically distinct DNA ligases, termed ligases I-IV, have been identified in mammalian cells. DNA ligase I is functionally homologous to the DNA ligase encoded by the Saccharomyces cerevisiae Cdc9 gene. The joining of Okazaki fragments during lagging strand DNA replication in mammalian cells is due to DNA ligase I. A combination of DNA polymerase epsilon, PCNA, replication factor C, replication protein A, and DNA ligase I is well-suited to the task of creating nucleotide excision repair patches.
• Synonyms: LIG1, LIG-1, DNA ligase 1, EC=6.5.1.1, DNA ligase I

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair, DNA replication, Mismatch repair, Nucleotide excision repair