alpha 2a Adrenergic Receptor (ADRA2A) Rabbit Polyclonal Antibody

CAT#: AP20407PU-N

alpha 2a Adrenergic Receptor (ADRA2A) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of AR alpha2A antibody (Cat.-No.: AP20407PU-N) in extracts from Jurkat cells.

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  Immunohistochemistry (IHC) analyzes of AR alpha2A antibody (Cat.-No.: AP20407PU-N) in paraffin-embedded human testis tissue.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: Western blot: 1/500-1/1000.
  Immunofluorescence: 1/50-1/200.
  Immunohistochemistry on Paraffin Sections: 1/50-1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of AR alpha-2A protein.
  (region surrounding Arg361)
• Formulation: Phosphate buffered saline (PBS), pH~7.2
  State: Aff - Purified
  State: Liquid purified Ig fraction (> 95% pure by SDS-PAGE).
  Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purification: Affinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~48 kDa
• Gene Name: adrenoceptor alpha 2A
• Database Link:
  Entrez Gene 11551 MouseEntrez Gene 25083 RatEntrez Gene 150 Human
  UniProt ID P08913
• Background: This study investigates the involvement of alpha2-adrenergic receptors (AR) in mouse brain induced by a low dose of methamphetamine (METH, 2 mg/kg). Immunohistochemical studies show that alpha2A-AR increased in the dentate gyrus area of the hippocampus 24 h after five repeated administrations of METH. The hippocampal alpha2A-AR proteins rose 3.2-fold when compared to the saline-administered mice. The other adrenergic receptor, apha1D-AR, were not changed by the treatment. Moreover, alphao-subunits of GTP-binding proteins (Galphao), one of the downstream molecules of alpha2A-AR, was also increased by the treatment. These suggest that the repeated administration of low-doses of METH causes quantitative changes of the signaling of alpha2A-AR in the mouse hippocampus.
• Synonyms: ADRA2A, ADRA2R, ADRAR, Alpha-2A adrenoreceptor, Alpha-2A adrenoceptor

Reference Data

• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction