GRK3 Rabbit Polyclonal Antibody

CAT#: AP20381PU-N

GRK3 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot (WB) analysis of GRK 3 antibody (Cat.-No.: AP20381PU-N) in extracts from HepG2 cells.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western Blot: 1/500 - 1/1000.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Specificity: This antibody detects endogenous levels of GRK 3 protein.
  (region surrounding Phe391)
• Formulation: Phosphate buffered saline (PBS) with 0.05% sodium azide, approx. pH 7.2.
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: 1.0 mg/ml
• Purification: Affinity chromatography (> 95% (by SDS-PAGE)
• Conjugation: Unconjugated
• Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Predicted Protein Size: ~ 80 kDa
• Gene Name: G protein-coupled receptor kinase 3
• Database Link:
  Entrez Gene 25372 RatEntrez Gene 320129 MouseEntrez Gene 157 Human
  UniProt ID P35626
• Background: Heterotrimeric G protein-mediated signal transduction is a dynamically regulated process with the intensity of signal decreasing over time despite the continued presence of the agonist. This phenomenon, referred to as agonistmediated desensitization, involves phosphorylation of the receptor by two classes of enzymes. The first are the second essenger-regulated kinases such as c-AMP dependent protein kinase A and protein kinase C. The second are the G protein-coupled receptor kinases (GRKs). At least seven members of the GRK family have been identified. These include rhodopsin kinase, GRK 1; two forms of beta-adrenergic receptor kinase, GRK 2 (betaARK, betaARK1) and GRK 3 (betaARK2); IT-11 (GRK 4); GRK 5, GRK 6 and GRK 7.
• Synonyms: Beta-adrenergic receptor kinase 2, BARK2, GRK3

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Chemokine signaling pathway, Endocytosis, Olfactory transduction