Aminomethyltransferase (AMT) (N-term) Rabbit Polyclonal Antibody

CAT#: AP17103PU-N

Aminomethyltransferase (AMT) (N-term) rabbit polyclonal antibody

Product Images

Western blot analysis of AMT Antibody (N-term) in HepG2 cell line lysates (35 ug/lane). AMT (arrow) was detected using the purified Pab.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blot: 1:50 - 1:100.
  ELISA: 1:1,000.
• Reactivities: Human
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the N-terminal region of human AMT
• Specificity: This antibody detects Aminomethyltransferase at N-term.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: aminomethyltransferase
• Database Link:
  Entrez Gene 275 Human
  UniProt ID P48728
• Background: The enzyme system for cleavage of glycine (glycine cleavage system; EC 2.1.2.10), which is confined to the mitochondria, is composed of 4 protein components: P protein (a pyridoxal phosphate-dependent glycine decarboxylase; MIM 238300), H protein (a lipoic acid-containing protein; MIM 238330), T protein (a tetrahydrofolate-requiring enzyme), and L protein (a lipoamide dehydrogenase; MIM 238331). Glycine encephalopathy (GCE; MIM 605899) may be due to a defect in any one of these enzymes.
• Synonyms: AMT, GCST, GCVT
• Note: Molecular weight: 43946 Da

Reference Data

• Protein Pathways: Glycine, serine and threonine metabolism, Metabolic pathways, Nitrogen metabolism, One carbon pool by folate