Albumin (ALB) (C-term) Rabbit Polyclonal Antibody

CAT#: AP17100PU-N

Albumin (ALB) (C-term) rabbit polyclonal antibody

Product Images

Western blot analysis of ALB antibody (C-term) in mouse liver tissue lysates (35 ug/lane). ALB (arrow) was detected using the purified Pab.

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  All lanes : Anti-ALB Antibody (C-term) at 1:1000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Hela whole cell lysate Lane 3: Mouse liver tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 69 kDa Blocking/Dilution buffer: 5% NFDM/TBST.

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  Immunohistochemical analysis of paraffin-embedded Human liver tissue using AP17100PU-N performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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  Immunohistochemical analysis of paraffin-embedded Human kidney tissue using AP17100PU-N performed on the Leica® BOND RXm. Tissue was fixed with formaldehyde at room temperature, antigen retrieval was by heat mediation with a EDTA buffer (pH9. 0). Samples were incubated with primary antibody(1:500) for 1 hours at room temperature. A undiluted biotinylated CRF Anti-Polyvalent HRP Polymer antibody was used as the secondary antibody.

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  ALB Antibody (C-term) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

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Specifications

Product Data

• Applications: FC, IHC, WB
• Recommended Dilution: Western blot: 1:1000
  IHC-P: 1:500
  FC: 1:10-1:50
• Reactivities: Human, Mouse (Predicted: Horse, Monkey)
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from 540-569 aa the C-terminal region of human ALB.
• Specificity: This antibody detects Albumin at C-term.
• Formulation: Supplied in PBS with 0.09% (W/V) sodium azide
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: albumin
• Database Link:
  Entrez Gene 11657 MouseEntrez Gene 704892 MonkeyEntrez Gene 213 Human
  UniProt ID P02768
• Background: Albumin is a soluble, monomeric protein which comprises about one-half of the blood serum protein. Albumin functions primarily as a carrier protein for steroids, fatty acids, and thyroid hormones and plays a role in stabilizing extracellular fluid volume. Albumin is a globular unglycosylated serum protein of molecular weight 65,000. Albumin is synthesized in the liver as preproalbumin which has an N-terminal peptide that is removed before the nascent protein is released from the rough endoplasmic reticulum. The product, proalbumin, is in turn cleaved in the Golgi vesicles to produce the secreted albumin.
• Synonyms: ALB, BSA, HSA, Serum Albumin
• Note: Molecular weight: 69367 Da

Reference Data

• Protein Families: Secreted Protein