ptch2 Goat Polyclonal Antibody

CAT#: AP16952PU-N

ptch2 goat polyclonal antibody, Aff - Purified

Specifications

Product Data

• Applications: ELISA, IF
• Recommended Dilution: Peptide ELISA: Antibody Detection Limit Dilution: 1/32000.
  Immunofluorescence studies of Zebrafish tumors: 1-3 µg/ml (Ju et al, Mol Cancer. 2009 Jun 25;8:40. PMID: 19555497).
• Reactivities: Zebrafish
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_571063.1
• Specificity: This antibody recognizes internal PTCH1.
• Formulation: Tris saline, pH 7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer.
  State: Aff - Purified
  State: Liquid purified IgG fraction.
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: patched 2
• Database Link:
  Entrez Gene 30181 Zebrafish
  UniProt ID Q98864
• Background: PTCH (Patched protein homolog 1) is a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). PTCH associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal.PTCH has a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis. PTCH is expressed in the adult brain, lung, liver, heart, placenta, skeletal muscle, pancreas and kidney. It is also expressed in tumor cells but not in normal skin. During development PTCH is found in all major target tissues of sonic hedgehog, such as the ventral neural tube, somites, and tissues surrounding the zone of polarizing activity of the limb bud.
  Defects in PTCH are probably the cause of basal cell nevus syndrome also known as Gorlin syndrome or Gorlin-Goltz syndrome.
• Synonyms: PTCH, PTC1, PTC, Patched 1