Synaptotagmin 1 (SYT1) Goat Polyclonal Antibody

CAT#: AP16929PU-N

Synaptotagmin 1 (SYT1) goat polyclonal antibody, Aff - Purified

Product Images

Antibody (1ug/ml) staining of Human Hippocampus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  SYT1 antibody staining of paraffin embedded Human Brain at 3.8 ug/ml. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Specifications

Product Data

• Applications: ELISA, IHC, WB
• Recommended Dilution: Peptide ELISA: Detection Limit: 1/16000.
  Western Blot: 0.3-1 µg/ml. Approx. 65kDa band observed in Human Brain (hippocampus) and Mouse and Rat Brain lysates (calculated MW of 47.6kDa according to NP_005630.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Matthew et al, J Cell Biol. 1981 Oct;91(1):257-69.; PMID: 7298720).
  Immunohistochemistry on Paraffin Sections: 4-6 µg/ml.
  In paraffin embedded Human Brain shows vesicular staining in neuronal cell and axon.
• Reactivities: Bovine, Canine, Human, Mouse, Rat
• Host: Goat
• Clonality: Polyclonal
• Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_005630.1.
• Specificity: This antibody recognizes Synaptotagmin I.
• Formulation: Tris saline, pH~7.3 containing 0.02% Sodium Azide as preservative and 0.5% BSA as stabilizer
  State: Aff - Purified
  State: Liquid purified Ig fraction
• Concentration: lot specific
• Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: synaptotagmin 1
• Database Link:
  Entrez Gene 6857 Human
  UniProt ID P21579
• Synonyms: SYT1, SVP65, SYT, Synaptotagmin1, Synaptotagmin I, SytI, p65