14-3-3 zeta (YWHAZ) Rabbit Polyclonal Antibody

CAT#: AP15151PU-N

14-3-3 zeta (YWHAZ) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of anti-14-3-3 protein zeta/delta Anbtibody (T232) in A375 cell line lysates (35ug/lane). 14-3-3 (arrow) was detected using the purified Pab (1:60dilution).

• 

  Formalin-fixed and paraffin-embedded human testis tissue reacted with PLD1 antibody (N-term) which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/50 - 1/100.
  Immunohistochemistry: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding T232 of human 14-3-3.
• Specificity: This antibody reacts to 14-3-3 protein zeta/delta.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein A column, followed by peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein zeta
• Database Link:
  Entrez Gene 7534 Human
  UniProt ID P63104
• Background: 14-3-3 protein zeta/delta belongs to the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. This protein interacts with IRS1 protein, suggesting a role in regulating insulin sensitivity. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse, rat and sheep orthologs.
• Synonyms: YWHAZ, KCIP-1