IARS2 Rabbit Polyclonal Antibody

CAT#: AP14675PU-N

IARS2 rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-IARS2 Antibody (Center) in 293 cell line lysates (35ug/lane).IARS2 (arrow) was detected using the purified Pab (1:60dilution).

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  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with IARS2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blotting: 1/50 - 1/100.
  Immunohistochemistry: 1/10 - 1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the Center region of human IARS2.
• Specificity: This antibody reacts to IARS2.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: isoleucyl-tRNA synthetase 2, mitochondrial
• Database Link:
  Entrez Gene 55699 Human
  UniProt ID Q9NSE4
• Background: IARS2 belongs to the class-I aminoacyl-tRNA synthetase family. Members of class I have two highly conserved sequence motifs. They aminoacylate at the 2'-OH of an adenosine nucleotide, and are usually monomeric or dimeric (one or two subunits, respectively). Both classes of aminoacyl-tRNA synthetases are multidomain proteins. The catalytic domains of all the aaRSs of a given class are found to be homologous to one another, while class I and class II aaRSs are unrelated to one another. The class I aaRSs have the ubiquitous Rossmann fold and have the antiparallel beta-strands architecture while the class II aaRSs have a unique fold made up of antiparallel beta-strands.
• Synonyms: mitochondrial Isoleucine-tRNA ligase, IleRS