MEK1 (MAP2K1) pSer222 Rabbit Polyclonal Antibody

CAT#: AP13022PU-N

MEK1 (MAP2K1) pSer222 rabbit polyclonal antibody, Aff - Purified

Product Images

Dot blot analysis of anti-Phospho-MEK1-pS222 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Specifications

Product Data

• Recommended Dilution: ELISA: 1/1,000.
  Dot Blot: 1/500.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S222 of human MEK1.
• Specificity: This antibody reacts to MEK1 pSer222.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Protein A column, followed by two-step phosphospecific peptide affinity purification
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: mitogen-activated protein kinase kinase 1
• Database Link:
  Entrez Gene 5604 Human
  UniProt ID Q02750
• Background: MEK1is a dual specificity protein kinase that belongs to the MAP kinase kinase family. This kinase is known to play a critical role in mitogen growth factor signal transduction. It phosphorylates and thus activates MAPK1/ERK2 and MAPK2/ERK3. The activation of this kinase itself is dependent on the Ser/Thr phosphorylation by MAP kinase kinase kinases. The inhibition or degradation of this kinase is found to be involved in the pathogenesis of Yersinia and anthrax.
• Synonyms: MAPKK 1, ERK activator kinase 1, MAPK/ERK kinase 1, MEK1, PRKMK1, MAP kinase kinase 1