FGFR1 pTyr766 Rabbit Polyclonal Antibody

CAT#: AP12611PU-N

FGFR1 pTyr766 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot analysis of anti-FGFR1-pY766 Phospho-specific Pab in Mouse cerebellum cell line lysate. Phospho-FGFR-Y766 (arrow) was detected using the purified Pab.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry. Clinical relevance has not been evaluated.

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  Dot blot analysis of anti-FGFR1-pY766 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Dot Blot: 1/500.
  Western Blot: 1/250-1/500.
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Tyr766 of Human FGFR.
• Specificity: This antibody detects FGFR pTyr766.
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: fibroblast growth factor receptor 1
• Database Link:
  Entrez Gene 2260 Human
  UniProt ID P11362
• Background: FGFR is a member of the fibroblast growth factor receptor family, where amino acid sequence is highly conserved between members and throughout evolution. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds both acidic and basic fibroblast growth factors and is involved in limb induction. Mutations in this gene can lead to Pfeiffer syndrome and Jackson-Weiss syndrome.
• Synonyms: BFGFR, CEK, FGFBR, FLG, FLT2, HBGFR, BFGFR, bFGF-R-1, FLT-2, N-sam, Proto-oncogene c-Fgr
• Note: Molecular weight: 91868 Da