APE1 (APEX1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP12510PU-N

APE1 (APEX1) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-rified Pab.

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  Western blot analysis of APEX1 (arrow) using APEX1 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the APEX1 gene.

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  Confocal Immunofluorescent analysis of APEX1 Antibody (N-term) with hela cell followed by Alexa Fluor 488-conjugated Goat anti-Rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/50-1/100.
  Immunofluorescence: 1/10-1/50.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide between 40~70 amino acids from the N-terminal region of Human APEX1
• Specificity: This antibody detects APEX1 / REF-1 (N-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: apurinic/apyrimidinic endodeoxyribonuclease 1
• Database Link:
  Entrez Gene 328 Human
  UniProt ID P27695
• Background: Apurinic/apyrimidinic (AP) sites occur frequently in DNA molecules by spontaneous hydrolysis, by DNA damaging agents or by DNA glycosylases that remove specific abnormal bases. AP sites are pre-mutagenic lesions that can prevent normal DNA replication so the cell contains systems to identify and repair such sites. Class II AP endonucleases cleave the phosphodiester backbone 5' to the AP site. This protein is the major AP endonuclease in human cells.
• Synonyms: APE, APEX, APX, HAP1, REF1, APEN
• Note: Molecular weight: 35555 Da