CD90 (THY1) (N-term) Rabbit Polyclonal Antibody

CAT#: AP11926PU-N

CD90 (THY1) (N-term) rabbit polyclonal antibody, Purified

Product Images

Figure 1. (LEFT) The anti-THY1 N-term Pab is used in Western blot to detect THY1 in HL60 cell lysate. (RIGHT) Western blot analysis of anti-THY1 (N-term) Pab pre-incubated without (lane 1) and with (lane 2) blocking peptide in T47D cell line lysate. THY1 (N-term) (arrow) was detected using the purified Pab (1:60 dilution).

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  Figure 2. (Top) Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma. (Buttom) Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with THY1 antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000
  Western blot: 1/100-1/500
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: Synthetic peptide selected from the N-terminal region of human CD90.
• Specificity: This antibody detects THY1/CD90 (N-term).
• Formulation: PBS with 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: Thy-1 cell surface antigen
• Database Link:
  Entrez Gene 7070 Human
  UniProt ID P04216
• Background: THY1 (CD90) antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain.
• Synonyms: Thy-1, THY1, CDw90
• Note: Calculated Molecular weight: 17935 Da