Stanniocalcin 2 (STC2) (N-term) Rabbit Polyclonal Antibody

CAT#: AP11418PU-N

Stanniocalcin 2 (STC2) (N-term) rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-STC2 Antibody (N-term) in mouse kidney tissue lysates (35 ug/lane). STC2 (arrow) was detected using the purified polyclonal antibody.

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  Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with STC2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA1: 1,000.
  Western blotting 1:100 - 1:500.
  Immunohistochemistry: 1:50 - 1:100.
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: KLH conjugated synthetic peptide selected from the N-terminal region of human STC2
• Specificity: This antibody detects STC2 at N-term.
• Formulation: PBS with 0.09% (W/V) sodium azide
  State: Purified
  State: Liquid Ig fraction
• Concentration: lot specific
• Purification: Protein A column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: stanniocalcin 2
• Database Link:
  Entrez Gene 8614 Human
  UniProt ID O76061
• Background: STC2 is a secreted, homodimeric glycoprotein that is expressed in a wide variety of tissues and may have autocrine or paracrine functions. The encoded protein has 10 of its 15 cysteine residues conserved among stanniocalcin family members and is phosphorylated by casein kinase 2 exclusively on its serine residues. Its C-terminus contains a cluster of histidine residues which may interact with metal ions. The protein may play a role in the regulation of renal and intestinal calcium and phosphate transport, cell metabolism, or cellular calcium/phosphate homeostasis. Constitutive overexpression of human stanniocalcin 2 in mice resulted in pre- and postnatal growth restriction, reduced bone and skeletal muscle growth, and organomegaly. Expression is induced by estrogen and altered in some breast cancers.
• Synonyms: Stanniocalcin-2, STC-2, Stanniocalcin-related protein, STC-related protein, STCRP
• Note: Molecular weight: 33249 Da