GST-Tag Rabbit Polyclonal Antibody

CAT#: AP11290PU-N

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GST-Tag rabbit polyclonal antibody, Purified



USD 512.00


Availability*
2 Weeks

Size
    • 400 ul


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Specifications

Product Data
Applications IHC, WB
Recommended Dilution ELISA: 1/1,000.
Western blot: 1/100-1/500
Immunohistochemistry: 1/50-1/100.
Reactivities Human
Host Rabbit
Isotype Ig
Clonality Polyclonal
Immunogen This antibody is generated from rabbits immunized with a recombinant full length of GST.
Specificity This antibody is specific to GST Tag.
Formulation PBS containing 0.09% (W/V) Sodium Azide as preservative.
State: Purified
State: Liquid purified Ig fraction.
Concentration lot specific
Purification Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Conjugation Unconjugated
Storage Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Stability Shelf life: one year from despatch.
Background Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
Synonyms Glutathione S-transferase Tag, GST26-Tag
Note Predicted MW: 25000 Da.
Reference Data
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*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.