GST-Tag Rabbit Polyclonal Antibody

CAT#: AP11290PU-N

GST-Tag rabbit polyclonal antibody, Purified

Product Images

Western Blot analysis using anti-GST Pab (Cat. #AP11290PU-N) to detect a GST-fusion recombinant protein (42 kDa) purified from bacterial lysate (Lanes 1-3: 10, 40, and 160 ng GST-fusion protein).

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western blot: 1/100-1/500
  Immunohistochemistry: 1/50-1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a recombinant full length of GST.
• Specificity: This antibody is specific to GST Tag.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P08515
• Background: Glutathione S-transferase (GST) was originally cloned from parasite Schistosoma japonicum and it is now a widely used protein fusion partner. Vectors containing GST Tag have been developed for both prokaryotic and eukaryotic systems. The GST fusion proteins are easily purified from cell lysates by affinity chromatography using Glutathione Sepharose 4B to elute out the GST fusion protein from the column with a denaturing form of glutathione. Using anti-GST antibody provides a simple solution to detect the expression of GST fusion proteins in cells.
• Synonyms: Glutathione S-transferase Tag, GST26-Tag
• Note: Predicted MW: 25000 Da.