DNMT3B Rabbit Polyclonal Antibody

CAT#: AP11052PU-N

DNMT3B rabbit polyclonal antibody, Purified

Product Images

Western blot analysis of anti-Dnmt3b Pab in T47-D cell lysate. Dnmt3b (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

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  Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

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  Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with Dnmt3b antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: ELISA: 1/1,000.
  Western Blot: 1/100-1/500.
  Immunohistochemistry: 1/10~1/100.
• Reactivities: Human
• Host: Rabbit
• Isotype: Ig
• Clonality: Polyclonal
• Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human Dnmt3b.
• Specificity: This antibody is specific to Dnmt3b.
• Formulation: PBS containing 0.09% (W/V) Sodium Azide as preservative.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: DNA (cytosine-5-)-methyltransferase 3 beta
• Database Link:
  Entrez Gene 1789 Human
  UniProt ID Q9UBC3
• Background: CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. Dnmt3b is a DNA methyltransferase which is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes primarily to the nucleus and its expression is developmentally regulated. Mutations in this gene cause the immunodeficiency-centromeric instability-facial anomalies (ICF)syndrome.
• Synonyms: DNA MTase HsaIIIB, M.HsaIIIB