SAE1 pSer185 Rabbit Polyclonal Antibody

CAT#: AP09416PU-S

SAE1 pSer185 rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot using Rabbit-anti-SAE1 pS185 antibody shows detection of phosphorylated SAE1. Left lane (-) contains 20 ug human HeLa whole cell protein. Right lane (+) contains 20 ug human HeLa whole cell protein from cells pre-treated with phosphatase inhibitor cocktail to prevent dephosphorylation of the target. Proteins were separated on a 10% SDS-PAGE and transferred onto nitrocellulose. After blocking with 5% milk-TBST 1 hr at room temperature, the membrane was probed with the primary antibody diluted to 1:1,000 at room temperature for 3 hr followed by washes and reaction with HRP-conjugated secondary and ECL imaging.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/100,000 - 1/125,000.
  Western Blot: 1/750 - 1/1,000.
• Reactivities: Bovine, Canine, Chimpanzee, Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to a region surrounding S185 of the human SUMO Activating Enzyme E1 protein
• Specificity: This antibody is directed against SUMO Activating Enzyme E1 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
  Shelf life: one year from despatch.
  
  Storage Conditions for Trial Size:
  This vial contains a relatively low volume of reagent (25 µl). To minimize loss of volume dilute 1:10 by adding 225 µl of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below.
• Stability: Shelf life: 3 month from despatch.
• Gene Name: SUMO1 activating enzyme subunit 1
• Database Link:
  Entrez Gene 10055 Human
  UniProt ID Q9UBE0
• Background: SUMO E1 activating enzyme (also called Ubiquitin-like 1 activating enzyme E1A, UBLE1A, AOS1, SAE1, and SUA1) with SAE2 (also known as UBA2) forms a heterodimeric (SAE1/SAE2) enzyme that activates the ubiquitinlike SUMO proteins (SUMO stands for Small Ubiquitin-like MOdifier.) The SAE1 (SUMO Activating Enzyme 1) subunit resembles the N-terminal half of yeast UBA1; the SAE2 (also called Uba2) subunit corresponds to the C-terminal part of yeast UBA1 and contains the active site cysteine. In the SUMO activation step, SAE1/SAE2 uses ATP to adenylate the C-terminal glycine of SUMO-1 (the first of the three different mammalian SUMO proteins) then forms a high-energy thioester bond between the C-terminal glycine and the active site cysteine in SAE2 (Uba2). In the conjugation step, the SUMO moiety is transferred from SAE1/SAE2 to the active site cysteine (Cys 93) of the SUMO conjugating enzyme (SUMO E2, Ubc9) forming a SUMO-E2 thioester complex.
• Synonyms: SUA1, UBLE1A