REC107 (26-35) Rabbit Polyclonal Antibody

CAT#: AP09317PU-N

REC107 (26-35) rabbit polyclonal antibody, Aff - Purified

Product Images

Western blot using affinity purified anti-S.cerevisiae Mer2 antibody shows detection of phosphorylated and unphosphorylated Mer2 in wild type, phosphatase treated and mutant cells. Lane 1 contains Mer2-myc protein detected in wild type cells after first immunoprecipitating the protein using anti-myc antibody. Cells were harvested 4 h after the initiation of meiosis and therefore contain mostly phosphorylated Mer2. Lane 2 contains the same preparation after treatment with phosphatase. Lane 3 contains Mer2-S30A protein as a phosphorylation control. This antibody is reactive with both phosphor-ylated and unphosphorylated Mer2 at the S30 position. The primary antibody was used at a 1:5,000 dilution.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA: 1/5,000 - 1/25,000.
  Western Blot: 1/1,000 - 1/10,000.
• Reactivities: Saccharomyces cerevisiae
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide corresponding to amino acids 26-35 of Saccharomyces cerevisiae Mer2 protein
• Specificity: This antibody is directed against the Saccharomyces cerevisiae Mer2 protein.
• Formulation: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01% (w/v) Sodium Azide
  State: Aff - Purified
  State: Liquid purified Ig
• Concentration: lot specific
• Purification: Affinity chromatography
• Conjugation: Unconjugated
• Storage: Store the antibody at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Database Link:
  UniProt ID P21651
• Background: Mer2 (also known as meiotic recombination 2 protein) is a chromosomal protein that is critical for meiotic recombination and progression. It is phosphorylated at two serine residues, S30 and S271 by the yeast Cdk1 cyclin- dependent kinase homolog. This phosphorylation is S-phase specific, and thus has the potential to be a specific assay for S-phase cyclin-dependent kinases. Moreover, there are hints that the phosphorylation may be a mark of replication fork passage, which would indicate that Sphase CDK associates with the replication fork.
• Synonyms: Meiotic recombination 2 protein, REC107, YJR021C, J1462