Horseradish Peroxidase / HRP Rat Polyclonal Antibody

CAT#: AP09100AF-N

Horseradish Peroxidase / HRP rat polyclonal antibody, Azide Free

Specifications

Product Data

• Applications: ELISA, IP, WB
• Recommended Dilution: Western Blot: 1/200 - 1/1000.
  Dot Blot.
  ELISA: 1/2000 - 1/6000.
  Immunoprecipitation.
• Host: Rat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Peroxidase [Horseradish]
• Specificity: This antibody reacts to Peroxidase [Horseradish].
• Formulation: 0.01 M Sodium Phosphate, 0.14 M Sodium Chloride, pH 7.4
  State: Azide Free
  State: Lyophilized
• Reconstitution Method: Restore with 2.0 ml of deionized water (or equivalent).
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Prior to reconstitution store at 2-8°C.
  Following reconstitution store the antibody undiluted at 2-8°C for one month
  or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Background: Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems.
  HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.