alpha 1 Fetoprotein (AFP) Rabbit Polyclonal Antibody

CAT#: AP08129PU-S

alpha 1 Fetoprotein (AFP) rabbit polyclonal antibody, Purified

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Suitable for Immunohistochemistry and Immunocytochemistry (Frozen or Formalin-Fixed Paraffin-Embedded (FFPE) tissue sections and cell smears): 1/50-1/100 using streptavidin~biotin system or polymer system.
  Recommended positive control: Human fetal liver, tonsil.
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Purified human GFAP.
• Specificity: AFP is a useful marker for detection of numerous developmental defects (open neural tube defect), hepatocarcinoma and endodermal sinus tumor (germ cell and yolk sac). Traces of AFP are found in normal adult sera and in greater concentrations in maternal, fetal and amniotic fluid.
  Cellular Localization: Cytoplasmic.
• Formulation: PBS, pH 7.4 containing 0.05% Sodium Azide as preservative and 1% BSA as stabilizer.
  State: Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: alpha fetoprotein
• Database Link:
  Entrez Gene 174 Human
  UniProt ID P02771
• Background: Alpha 1 Fetoprotein is a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. Expression has been documented in human adrenal, liver, ovary, testis, and pancreas. ESTs have been isolated from normal human brain, liver/spleen, embryo and uterus tissue libraries.
• Synonyms: Alpha-1-fetoprotein, Alpha-fetoglobulin, HPAFP