SMAD2 Rabbit Polyclonal Antibody

CAT#: AP08072PU-S

SMAD2 rabbit polyclonal antibody, Aff - Purified

Product Images

Western Blot analysis of extracts from HeLa cells using Smad2 antibody

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  Immunofluorescence staining of methanol-fixed HeLa cells using SMAD2 antibody (Red).

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: Western blot: 1/500 - 1/1000.
  Immunofluorescence: 1/100 - 1/200.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized non-phosphopeptide derived from human SMAD2 around the phosphorylation site of Serine 467 (C-S-S-M-Sp)
• Specificity: This antibody detects endogenous levels of total SMAD2 protein.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Immunoaffinity Chromatography using epitope-specific immunogen.
• Conjugation: Unconjugated
• Storage: Upon receipt, store undiluted (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: one year from despatch.
• Gene Name: SMAD family member 2
• Database Link:
  Entrez Gene 4087 Human
  UniProt ID Q15796
• Background: Smad2 is a member of the Smad family of proteins which are similar to the gene products of the Drosophila gene 'mothers against decapentaplegic' (Mad) and the C. elegans gene Sma. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. This protein mediates the signal of the transforming growth factor (TGF)-beta, and thus regulates multiple cellular processes, such as cell proliferation, apoptosis, and differentiation. This protein is recruited to the TGF-beta receptors through its interaction with the SMAD anchor for receptor activation (SARA) protein. In response to TGF-beta signal, this protein is phosphorylated by the TGF-beta receptors. The phosphorylation induces the dissociation of this protein with SARA and the association with the family member SMAD4. The association with SMAD4 is important for the translocation of this protein into the nucleus, where it binds to target promoters and forms a transcription repressor complex with other cofactors. This protein can also be phosphorylated by activin type 1 receptor kinase, and mediates the signal from the activin. Alternatively spliced transcript variants encoding the same protein have been observed.
• Synonyms: SMAD family member 2, SMAD-2, SMAD 2, MADH2, MAD homolog 2, MADR2, Mad-related protein 2, JV18-1