AKT1 pTyr315/316/312 Rabbit Polyclonal Antibody

CAT#: AP08030PU-N

AKT1 pTyr315/316/312 rabbit polyclonal antibody, Aff - Purified

Product Images

Figure 1. Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312) Antibody.

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  Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using AKT1/AKT2/AKT3 (Phospho-Tyr315/316/312) Antibody (Red).

Specifications

Product Data

• Applications: IF, IHC
• Recommended Dilution: Immunofluorescence: 1/100-1/200.
  Immunohistochemistry on Paraffin-Embedded Sections: 1/50-1/100.
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: The antiserum was produced against synthesized phosphopeptide derived from human AKT1/AKT2/AKT3 around the phosphorylation site of Tyrosine 315/316/312 (P-E-YP-L-A).
• Specificity: This Antibody AP08030PU detects endogenous levels of AKT1/AKT2/AKT3 only when phosphorylated at Tyrosine 315/316/312.
• Formulation: PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02% Sodium Azide and 50% Glycerol.
  State: Aff - Purified
  State: Liquid purified Ig fraction.
• Concentration: lot specific
• Purification: Affinity Cchromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
• Conjugation: Unconjugated
• Storage: Store the antibody (in aliquots) at -20°C.
  Avoid repeated freezing and thawing.
• Stability: Shelf life: One year from despatch.
• Gene Name: AKT serine/threonine kinase 1
• Database Link:
  Entrez Gene 207 Human
  UniProt ID P31749
• Background: AKT1/2/3 are members of the AKT, also called PKB, serine/threonine protein kinase family. AKT kinases are known to be regulators of cell signaling in response to insulin and growth factors. They are involved in a wide variety of biological processes including cell proliferation, differentiation, apoptosis, tumorigenesis, as well as glycogen synthesis and glucose uptake.
• Synonyms: Akt-1, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma, PKB gamma, Protein kinase B, C-AKT, PKBG, AKT-2, AKT-3 Review 010